Feulgen Reaction and Quantitative Cytochemistry of Desoxypentose Nucleic Acid
نویسندگان
چکیده
منابع مشابه
Reminiscences about nucleic acid cytochemistry and biochemistry
There was sharp opposition between ‘analomists and ‘physiologists’ when I was a medical student in the University of Urussels, some 60 years ago. This split was cxcmplificd by the prcsencc of two separate buildings. called respcctivcly Institutes of Anatomy and of Physiology, in the newly crcctcd Medical School. The first housed embryologists, histologists and pathologists, the second physiolog...
متن کاملQuantitative Cytochemical Determination of Desoxyribonucleic Acid with the Feulgen Nucleal Reaction
The possibility of using the Feulgen nucleal reaction for a quantitative cytochemical estimation of desoxyribonucleic acid (DNA) was investigated. The intensity of the reaction in nuclei was determined by absorption measurements with the microscope. The accuracy of such measurements was tested by comparison with measurements on the same material with a Beckman spectrophotometer. The values obta...
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The distribution of purines and pyrimidines in desoxypentose nucleic acids prepared from a variety of animal and plant sources has been studied. 1. The nucleic acids were prepared from calf thymus, calf kidney, sheep spleen, horse spleen, chicken erythrocyte, turtle erythrocyte, trout sperm, shad testes, sea urchin sperm, wheat germ, and Pneumococcus Type III. 2. Separate hydrolyses were carrie...
متن کاملFEULGEN NUCLEAL REACTION I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCL*OPROTEII~ BY TOSISUKE HIRAOKA, D.Sc
Recent advances in cytochemistry have revealed that two types of nucleic acids play important roles in the cell (for references, 1 and 2). Whereas these two types of nucleic acids, deoxypentose nucleic acid (DNA) and pentose nucleic acid (PNA), have similar ultraviolet absorption properties, they are distinguished from each other by certain color reactions based on their constituent sugars. Amo...
متن کاملFEULGEN NUCLEAL REACTION I. QUANTITATIVE EXTRACTION OF FUCHSIN FROM FEULGEN-STAINED NUCL*OPROTEII~ BY TOSISUKE HIRAOKA, D.Sc
Recent advances in cytochemistry have revealed that two types of nucleic acids play important roles in the cell (for references, 1 and 2). Whereas these two types of nucleic acids, deoxypentose nucleic acid (DNA) and pentose nucleic acid (PNA), have similar ultraviolet absorption properties, they are distinguished from each other by certain color reactions based on their constituent sugars. Amo...
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ژورنال
عنوان ژورنال: CYTOLOGIA
سال: 1953
ISSN: 0011-4545,1348-7019
DOI: 10.1508/cytologia.18.1